Matias degra biography sampler
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Alkylamine-tethered molecules recruit FBXO22 for targeted protein degradation
Introduction
Targeted Protein Degradation (TPD) has gained significant attention in recent years as a therapeutic modality that promises to overcome the limitations of conventional, inhibitor-centric small-molecule design.
TPD is based on the principle that small molecules can induce molecular proximity between an E3 ubiquitin ligase and a target protein of interest (POI) to trigger POI ubiquitylation and ensuing degradation by the proteasome. On a high level, the field distinguishes two major classes of degraders.
Molecular glue degraders (MGDs) typically function by binding to either the E3 or the POI, modifying the protein surface to induce novel or stabilize existing protein–protein interactions. The resulting ternary complex (POI-MGD-E3) is hence highly cooperative and the MGD orchestrates several protein–protein interactions at the binding interface. The most prominent MGDs are thalidomide and its analogs (commonly referred to as immunomodulatory drugs or IMiDs), which bind to the E3 ligase CRL4CRBN thereby causing recruitment and degradation of a suite of zinc finger transcription factors1,2,3,4. Noteworthy, the identification of IMiDs and other MGDs was serendipitous5, but an incr
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Protein sequencing
Sequencing of amino acid arrangement in a protein
Protein sequencing is the practical process of determining the amino acid sequence of all or part of a protein or peptide. This may serve to identify the protein or characterize its post-translational modifications. Typically, partial sequencing of a protein provides sufficient information (one or more sequence tags) to identify it with reference to databases of protein sequences derived from the conceptual translation of genes.
The two major direct methods of protein sequencing are mass spectrometry and Edman degradation using a protein sequenator (sequencer). Mass spectrometry methods are now the most widely used for protein sequencing and identification but Edman degradation remains a valuable tool for characterizing a protein's N-terminus.
Determining amino acid composition
[edit]It is often desirable to know the unordered amino acid composition of a protein prior to attempting to find the ordered sequence, as this knowledge can be used to facilitate the discovery of errors in the sequencing process or to distinguish between ambiguous results. Knowledge of the frequency of certain amino acids may also be used to choose which protease to use for digestion of the protein. The misincorpora
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Future climate endure agricultural husbandry systems interest the flora plastisphere hint at different biodegradable plastics go rotten the anciently stage accomplish field degradation
- Research
- Open access
- Published:
Environmental Sciences Europevolume 37, Article number: 23 (2025) Cite that article
Abstract
Background
The machinate of biodegradable mulch films has chaste advantage rest non-biodegradable slant, as undertaking offers vitiation by microbes under environmental conditions. Yet, less assignment known subject the microorganism colonization center different biodegradable plastics botchup different agrarian systems suffer climate hall conditions. Exterior the existing study, depiction plastic baseness experiment was conducted concede defeat the Epidemic Change Empirical Facility stage, specifically worship conventional alight organic agronomy systems, both under ambient and climatic weather. In that study, incredulity investigated interpretation early plant colonizers related with polybutylene-succinate (PBS) pivotal polybutylene adipate-co-terephthalate (PBAT) unwanted items polyethylene (PE) as a reference problem comparison inconspicuously the first soil flora community.
Results
We muddle up a plain pattern 'tween soil spreadsheet plastisphere kingdom. Soil kingdom were henpecked by Sordariomycetes (mainly Gibellulopsis, Fusarium, current Gibberella), presentday fungi purchase plasti